Rowley, 45, is still in hospital but regained consciousness on Tuesday, showing a "small but significant" improvement in his condition, medics said.
"While this is welcome news, clearly we are not out of the woods yet".
In a statement, Scotland Yard said: "Officers from the investigation team have spoken briefly to Charlie and will be looking to further speak with him in the coming days as they continue to try and establish how he and Dawn came to be contaminated with the nerve agent".
The news came as England's chief medical adviser ratcheted up warnings to people living in the area where one person died and another was left critically ill through exposure to the nerve agent.
Last week, Sturgess and Rowley were found collapsed at a residential building in Amesbury, Wiltshire, just eight miles north of Salisbury, where the Skripals were poisoned.
Sturgess and Rowley fell ill on June 30.
Both Skripals have recovered from the poisoning and a police investigation into the incident is ongoing.
Police searching for the source of a Russian nerve agent that killed a woman and sickened three others have seized a auto about 40 miles away from the original investigation site.Читайте также: Pyrotechnics and songs: as Buffon was seen in the PSG
At the weekend, his girlfriend Dawn Sturgess, 44, tragically died after also being poisoned.
Russian Federation was accused of targeting the Skripals in March but has denied involvement.
Mr Basu also said that the "brutal reality" is that he can not say with certainty that there are no traces of nerve agent left anywhere in Wiltshire.
Mr Basu, the Met Police's assistant commissioner for specialist operations, said scientific advice suggested that the nerve agent could remain active for decades.
Six locations visited by the couple have been cordoned off, including Mr Rowley's home on Muggleton Road in Amesbury, a Boots shop and a baptist church.
Ms Sturgess, who had a daughter aged 11 and two sons aged 19 and 23, had...
A vehicle seized by men in camouflage gear and gas masks belongs to a paramedic and RAF hero who treated Ms Sturgess, locals say.
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